Mutation supply and the repeatability of selection for antibiotic resistance

Whether evolution can be predicted is a key question in evolutionary biology. Here we set out to better understand the repeatability of evolution. We explored experimentally the effect of mutation supply and the strength of selective pressure on the repeatability of selection from standing genetic variation. Different sizes of mutant libraries of an antibiotic resistance gene, TEM-1 $\beta$-lactamase in Escherichia coli, were subjected to different antibiotic concentrations. We determined whether populations went extinct or survived, and sequenced the TEM gene of the surviving populations. The distribution of mutations per allele in our mutant libraries- generated by error-prone PCR- followed a Poisson distribution. Extinction patterns could be explained by a simple stochastic model that assumed the sampling of beneficial mutations was key for survival. In most surviving populations, alleles containing at least one known large-effect beneficial mutation were present. These genotype data also support a model which only invokes sampling effects to describe the occurrence of alleles containing large-effect driver mutations. Hence, evolution is largely predictable given cursory knowledge of mutational fitness effects, the mutation rate and population size. There were no clear trends in the repeatability of selected mutants when we considered all mutations present. However, when only known large-effect mutations were considered, the outcome of selection is less repeatable for large libraries, in contrast to expectations. Furthermore, we show experimentally that alleles carrying multiple mutations selected from large libraries confer higher resistance levels relative to alleles with only a known large-effect mutation, suggesting that the scarcity of high-resistance alleles carrying multiple mutations may contribute to the decrease in repeatability at large library sizes.Comment: 31pages, 9 figure

LOAD EFFECTS OF FAST LONGITUDINAL ROTATIONS

Pirouettes, jumps in figure skating and twisting somersaults are fascinating movements in sports. But there is a lack of investigations in the field of motor control and load effects of fast longitudinal rotations. The purpose of the present study was to analyze load effects during and after longitudinal rotations. A "Longitudinal Rotation Simulator", posturography, and video nystagmography were used to analyze the rotational load. Six gymnasts, eleven figure skaters, and nineteen non-athletes were investigated. There were significant differences in the postural control between gymnasts and non-athletes. Per-rotatory nystagmus amplitude was significantly lower after three rotations than after ten rotations. The posturography and the video nystagmography are valid methods to characterize the rotational load

Spiral Growth and Step Edge Barriers

The growth of spiral mounds containing a screw dislocation is compared to the growth of wedding cakes by two-dimensional nucleation. Using phase field simulations and homoepitaxial growth experiments on the Pt(111) surface we show that both structures attain the same characteristic large scale shape when a significant step edge barrier suppresses interlayer transport. The higher vertical growth rate observed for the spiral mounds on Pt(111) reflects the different incorporation mechanisms for atoms in the top region and can be formally represented by an enhanced apparent step edge barrier.Comment: 11 pages, 4 figures, partly in colo

Towards Digital Twin-enabled DevOps for CPS providing Architecture-Based Service Adaptation & Verification at Runtime

Industrial Product-Service Systems (IPSS) denote a service-oriented (SO) way of providing access to CPS capabilities. The design of such systems bears high risk due to uncertainty in requirements related to service function and behavior, operation environments, and evolving customer needs. Such risks and uncertainties are well known in the IT sector, where DevOps principles ensure continuous system improvement through reliable and frequent delivery processes. A modular and SO system architecture complements these processes to facilitate IT system adaptation and evolution. This work proposes a method to use and extend the Digital Twins (DTs) of IPSS assets for enabling the continuous optimization of CPS service delivery and the latter's adaptation to changing needs and environments. This reduces uncertainty during design and operations by assuring IPSS integrity and availability, especially for design and service adaptations at CPS runtime. The method builds on transferring IT DevOps principles to DT-enabled CPS IPSS. The chosen design approach integrates, reuses, and aligns the DT processing and communication resources with DevOps requirements derived from literature. We use these requirements to propose a DT-enabled self-adaptive CPS model, which guides the realization of DT-enabled DevOps in CPS IPSS. We further propose detailed design models for operation-critical DTs that integrate CPS closed-loop control and architecture-based CPS adaptation. This integrated approach enables the implementation of A/B testing as a use case and central concept to enable CPS IPSS service adaptation and reconfiguration. The self-adaptive CPS model and DT design concept have been validated in an evaluation environment for operation-critical CPS IPSS. The demonstrator achieved sub-millisecond cycle times during service A/B testing at runtime without causing CPS operation interferences and downtime.Comment: Final published version appearing in 17th Symposium on Software Engineering for Adaptive and Self-Managing Systems (SEAMS 2022

Options for accounting carbon sequestration in German forests

<p>Abstract</p> <p>Background</p> <p>The Accra climate change talks held from 21–27 August 2008 in Accra, Ghana, were part of an ongoing series of meetings leading up to the Copenhagen meeting in December 2009. During the meeting a set of options for accounting carbon sequestration in forestry on a post-2012 framework was presented. The options include gross-net and net-net accounting and approaches for establishing baselines.</p> <p>Results</p> <p>This article demonstrates the embedded consequences of Accra Accounting Options for the case study of German national GHG accounting. It presents the most current assessment of sequestration rates by forest management for the period 1990 – 2007, provides an outlook of future emissions and removals (up to the year 2042) as related to three different management scenarios, and shows that implementation of some Accra options may reverse sources to sinks, or sinks to sources.</p> <p>Conclusion</p> <p>The results of the study highlight the importance of elaborating an accounting system that would prioritize the climate convention goals, not national preferences.</p

Magnetron sputtering fabrication of α-Al2O3:Cr powders and their T thermoluminescence properties

The authors gratefully acknowledge the financial support for this work from research grant ERA.NET RUS Plus Nr.609556.For many years doped α-Al2O3 has attracted interest as a dosimeter for personal, environment monitoring and food control. The alumina single crystal growth is a difficult process; however, materials in form of powders, ceramics and coating are possible to obtain. In this study for the first time Cr doped α-Al2O3 powders were prepared by DC reactive magnetron sputtering followed by milling and oxidation. The morphology and phase analysis was performed; content of residual impurities was determined and thermostimulated glow curves were measured after different x-ray irradiation times (radiation doses). The prepared powder shows the dosimetry properties up to 20 kGy dose.ERA.NET RUS Plus Nr.609556; Institute of Solid State Physics, University of Latvia as the Center of Excellence has received funding from the European Union’s Horizon 2020 Framework Programme H2020-WIDESPREAD-01-2016-2017-TeamingPhase2 under grant agreement No. 739508, project CAMART

Геолого-промышленные типы месторождений германия, методика поисков и разведки

Recent genetic studies found the A allele of the variant rs1006737 in the alpha 1C subunit of the L-type voltage-gated calcium channel (CACNA1C) gene to be overrepresented in patients suffering from bipolar disorder, schizophrenia or major depression. While the functions underlying the pathophysiology of these psychiatric disorders are yet unknown, impaired performance in verbal fluency tasks is an often replicated finding. We investigated the influence of the rs1006737 single nucleotide polymorphism (SNP) on verbal fluency and its neural correlates.Brain activation was measured with functional magnetic resonance imaging (fMRI) during a semantic verbal fluency task in 63 healthy male individuals. They additionally performed more demanding verbal fluency tasks outside the scanner. All subjects were genotyped for CACNA1C rs1006737.For the behavioral measures outside the scanner, rs1006737genotype had an effect on semantic but not on lexical verbal fluency with decreased performance in risk-allele carriers. In the fMRI experiment, while there were no differences in behavioural performance, increased activation in the left inferior frontal gyrus as well as the left precuneus was found in risk-allele carriers in the semantic verbal fluency task.The rs1006737 variant does influence language production on a semantic level in conjunction with the underlying neural systems. These findings are in line with results of studies in bipolar disorder, schizophrenia and major depression and may explain some of the cognitive and brain activation variation found in these disorders